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High Throughput Mapping of Wide-Scale Protein Phosphorylation Events in Cells based solely on Multi-dimensional Chromatography/ Mass Spectrometry Techniques

presented by

Scott Ficarro
Genomics Institute of Novartis Research

August 01, 2002

The Scripps Research Institute, W.M. Keck Foundation Amphitheater


Background:

Dr. Scott Ficarro received his B.S. in Biochemistry from Rider University in 1997, and his Ph.D. in Chemistry at the University of Virginia in 2001 in the laboratory of Prof. Donald F. Hunt. During his graduate studies, Dr. Ficarro developed novel methods for the isolation and sequencing of phosphorylated peptides in complex mixtures based solely on affinity chromatography combined with ESI-MS. In October of 2001, he joined the protein profiling group at the Genomics Institute of the Novartis Research Foundation, where he has continued to develop new methods for the identification of various protein post-translational modifications using liquid chromatography/ mass spectrometry techniques, as well as integrate these technologies into high throughput ESI and MALDI MS platforms for the rapid elucidation of cellular signaling cascades.

Abstract:

This talk describes a straightforward yet sensitive approach based solely on multi-dimensional liquid chromatography/ mass spectrometry that enables the fast, wide-scale identification of changes in numerous sites of phosphorylation on many different proteins from human whole cell lysates over time. In addition to performing "global" phosphorylation analyses, this technique can further be tailored to better focus on those phosphorylation events known to be involved in modulating important biological processes such as cellular signaling and differentiation. Several examples of using this methodology to follow phosphorylation events either during well-defined signaling cascades or upon perturbation of a system will be presented.

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