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Analysis of Intact Protein Complexes by MALDI-TOF Mass Spectrometry: Technology and Applications

presented by

Ryan Wenzel
Co-Founder and Chief Scientific Officer
CovalX GmbH

February 15, 2007

W.M. Keck Foundation Amphitheater


Background:


  • McPherson College, McPherson, KS., B.S.in Chemistry, 1994-1998,

  • University of California - Riverside, Riverside, CA M.S. in Chemistry, 1999

  • University of California - Riverside, Riverside, CA Ph.D.in Analytical Chemistry, 2002, Kim Prather (PI)

  • UC San Diego 2000-2002

  • Swiss Federal Institute of Technology (ETHZ), Z?rich, Switzerland, Postdoctoral Researcher, 2002-2005, Prof. Renato Zenobi

  • CovalX AG, Z?rich, Switzerland, 2005-, Co-Founder and Chief Scientific Officer

Abstract:

     There is increasing effort into the measurement of intact protein complexes by mass spectrometry. Most attempts have been through using ESI which allows detection of the high molecular weight complexes because of the multiple charges of the ions. MALDI is not routinely used because it is difficult for the complex to survive the MALDI preparation and then detection of the high mass singly charged molecules was often not possible. Presented is a novel method to measure protein complexes by MALDI mass spectrometry through first covalently stabilizing the complexes and then analyzing using a retrofitable high mass detector. Applications for this technique include immunology, protein pathway research, biopharmaceutical quality control and drug discovery. Various specific problems such as stoichiometry of complexes, kinetics of binding, identification of the partners, and disruption of complexes will be shown, demonstrating several specific biological problems that have been answered using this fast and effective technique.

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